Preliminary characterization of TRIM38. (A) Alignment of N-terminal domain TRIM proteins amino acid sequences by Vector NTI advance 9. (B) Evolutionary tree of TRIM family members encoded by genes on human chromosome 6p21. Amino acid sequences of TRIM family members were aligned using Clustal W. The alignment was then used to build trees in MEGA4. The scale bar represents evolutionary distance in amino acid residues. (C) TRIM38 was visualized via labeling with polyclonal rabbit anti-TRIM38, followed by labeling with anti-rabbit IgG/DyLight 594 conjugated secondary antibodies. Representative confocal images are shown.