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Table 3 HAdV prevalence in Hawaiian urban wastewater and environmental waters

From: Effective detection of human adenovirus in hawaiian waters using enhanced pcr methods

Sample

PCR

Nested PCR

  

primary

nested

Site

Condition

Volume

ADV-F ADV-R

nehex3deg nehex4deg

hex1deg hex2deg

hex1deg/hex2deg; nehex3deg/nehex4deg

SIWWTP influence tank

Sewage

100 mL

+

+

+

ND

SIWWTP clarifying tank

Sewage

100 mL

+

+

+

ND

SIWWTP effluence tank

Sewage

100 mL

+

+

+

ND

Sand Island State Recreational Area

Seawater

2.00 L

-

-

-

+

Kailua Bay

Seawater

2.00 L

+

-

-

+

Waikiki Beach

Seawater

2.00 L

-

+

-

+

Ala Wai Canal

Freshwater

2.00 L

-

-

-

+

Wahiawa freshwater

Freshwater

2.00 L

-

-

-

+

Manoa stream

Freshwater

2.00 L

+

+

-

+

Ala Moan Park/Magic Island

Seawater

2.00 L

-

-

-

-

Diamond Head Beach Park

Seawater

2.00 L

-

-

-

-

Maili Beach Park

Seawater

2.00 L

-

-

-

-

Waianae/Pokai Bay

Seawater

2.00 L

-

-

-

-

Waialae Beach Park

Seawater

2.00 L

-

-

-

-

Maunalua Bay Beach Park

Seawater

2.00 L

-

-

-

-

Kalaka Bay Beach Park

Seawater

2.00 L

-

-

-

-

Bellows Field Beach Park

Seawater

0.80 La

-

-

-

-

West Loch Community Shoreline Park

Seawater

0.80 La

-

-

-

-

Kailua Stream

Freshwater

0.50 La

-

-

-

-

Field Blank

dH2O

2.00 L

-

-

-

-

Spike control

S+Sb

2 +0.05 L

+

+

+

+

  1. a. 2L sample water was initially prepared but actual volume passed through the filter membrane.
  2. b. 2 L of seawater + 50 mL of sewage water.
  3. c. ND = not done