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Table 3 HAdV prevalence in Hawaiian urban wastewater and environmental waters

From: Effective detection of human adenovirus in hawaiian waters using enhanced pcr methods

Sample PCR Nested PCR
   primary nested
Site Condition Volume ADV-F ADV-R nehex3deg nehex4deg hex1deg hex2deg hex1deg/hex2deg; nehex3deg/nehex4deg
SIWWTP influence tank Sewage 100 mL + + + ND
SIWWTP clarifying tank Sewage 100 mL + + + ND
SIWWTP effluence tank Sewage 100 mL + + + ND
Sand Island State Recreational Area Seawater 2.00 L - - - +
Kailua Bay Seawater 2.00 L + - - +
Waikiki Beach Seawater 2.00 L - + - +
Ala Wai Canal Freshwater 2.00 L - - - +
Wahiawa freshwater Freshwater 2.00 L - - - +
Manoa stream Freshwater 2.00 L + + - +
Ala Moan Park/Magic Island Seawater 2.00 L - - - -
Diamond Head Beach Park Seawater 2.00 L - - - -
Maili Beach Park Seawater 2.00 L - - - -
Waianae/Pokai Bay Seawater 2.00 L - - - -
Waialae Beach Park Seawater 2.00 L - - - -
Maunalua Bay Beach Park Seawater 2.00 L - - - -
Kalaka Bay Beach Park Seawater 2.00 L - - - -
Bellows Field Beach Park Seawater 0.80 La - - - -
West Loch Community Shoreline Park Seawater 0.80 La - - - -
Kailua Stream Freshwater 0.50 La - - - -
Field Blank dH2O 2.00 L - - - -
Spike control S+Sb 2 +0.05 L + + + +
  1. a. 2L sample water was initially prepared but actual volume passed through the filter membrane.
  2. b. 2 L of seawater + 50 mL of sewage water.
  3. c. ND = not done