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Table 2 Optimized PCR conditions and detection limits for each primer set

From: Effective detection of human adenovirus in hawaiian waters using enhanced pcr methods

Primer Stda Optimized condition Detection limit
   Tannel [MgCl2] [primer]  
hex1deg/hex2deg 54-60 1.5 mM 600-800 nM 10-5 X
nehex3deg/nehex4deg 58-60 2.0 mM 600-800 nM 10-6-7 X
ADV-F/ADV-R 51.6-55.4 1.5 mM 600-1000 nM 10-7 X
XuHex1/XuHex2 54-60 1.5 mM 1000 nM 10-5 X
hexDEGF/hexDEGR 59.2 3.0 mM 600-800 nM 10-5 X
AdF/AdR 60 1.5 mM 600 nM 10-6-7 X
AdV1/AdV2 51.6-55.4 2.0 mM 600-800 nM 10-4X
AdV3ne/AdV4ne 55.4 1.5 mM 400-800 nM 10-4 X
Hex1deg/hex2deg; nehex3deg/nehex4degb    10-7 X
AdV1/AdV2; AdV3ne/AdV4neb    10-5 X
  1. a. Standard condition as described in text.
  2. b. Nested PCR.