Skip to main content

Table 2 Optimized PCR conditions and detection limits for each primer set

From: Effective detection of human adenovirus in hawaiian waters using enhanced pcr methods

Primer

Stda

Optimized condition

Detection limit

  

Tannel

[MgCl2]

[primer]

 

hex1deg/hex2deg

✓

54-60

1.5 mM

600-800 nM

10-5 X

nehex3deg/nehex4deg

✓

58-60

2.0 mM

600-800 nM

10-6-7 X

ADV-F/ADV-R

✓

51.6-55.4

1.5 mM

600-1000 nM

10-7 X

XuHex1/XuHex2

✓

54-60

1.5 mM

1000 nM

10-5 X

hexDEGF/hexDEGR

✓

59.2

3.0 mM

600-800 nM

10-5 X

AdF/AdR

✓

60

1.5 mM

600 nM

10-6-7 X

AdV1/AdV2

✓

51.6-55.4

2.0 mM

600-800 nM

10-4X

AdV3ne/AdV4ne

✓

55.4

1.5 mM

400-800 nM

10-4 X

Hex1deg/hex2deg; nehex3deg/nehex4degb

  

10-7 X

AdV1/AdV2; AdV3ne/AdV4neb

  

10-5 X

  1. a. Standard condition as described in text.
  2. b. Nested PCR.