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Table 4 Quantitative PCR of the target miRNA inhibiting ALV-J replication

From: Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs

Group

mRNA levels of target gene

mRNA levels of β-actin

mRNA levels of target gene by β-actin normalization

Rates of viral suppression (%)

p-values

mi-g1318-p2516

0.62 ± 0.17

4.59 ± 0.16

0.135

86.5*

0.01

mi-g1318-e1384

0.58 ± 0.11

3.87 ± 0.13

0.150

85.0*

0.01

mi-p2516-e1384

0.48 ± 0.10

4.02 ± 0.15

0.120

88.0*

0.01

mi-g1318-p2516-e1384

0.35 ± 0.09

3.98 ± 0.12

0.088

91.2*

0.01

Negative control

3.75 ± 0.18

3.68 ± 0.15

1.030

0

0

Vector control

3.18 ± 0.12

3.25 ± 0.10

0.987

0

0

  1. Statistically significant differences from the Negative control are indicated by*(P < 0.05)