Table 2 Primers used to amplify target genes
From: Enhanced inhibition of Avian leukosis virus subgroup J replication by multi-target miRNAs
Target gene (Accession number) | Primer sequence | Product size (bp) | Annealing Temperature (°e) |
|---|---|---|---|
Gag | Forward TCAGGACCAAGGGCTTAC | 174 | 55.2 |
| Â | Reverse CTGCCGCTATAACCGTCTG | Â | Â |
ALV specific primer | Forward TCAGGACCAAGGGCTTAC | 545 | 55.0 |
| Â | Reverse CTGCCGCTATAACCGTCTG | Â | Â |
β-actin | Forward TCCCTGTATGCCTCTGGTC | 250 | 55.0 |
| Â | Reverse TCTCTCTCGGCTGTGGTGG | Â | Â |