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Table 1 Primers for PCR in amplification of cDNA fragments of the three strains

From: Novel sequences of subgroup J avian leukosis viruses associated with hemangioma in Chinese layer hens

Segments

Primers

Sequences(from 5'- 3')

Product sizes/bp

W1

W1F

GGTGTAGTGTTATGCAATACTC

2782

 

W1R

ACAGGCGTGTGGTCTGGCTTCC

 

W2

W2F

GGACTGTTGCGCTACATCTGGC

2788

 

W2R

GACCCACACGTTTCCTGGTTG

 

W3

W3F

GTGCGTGGTTATTATTTCCGTT

2330

 

W3R

ACCAATGTGGTGGGAGGTAAA

 

W4

W4F

TTAGGAAGGCAACAGACGG

466

 

W4R

GGGCGACCAGAATCACG

 
  1. Primers W1, W2, W3 used to amplify the sequence from position 1 - 7841 of the prototype ALV-J strain HPRS-103. Primers W4 used to amplify the long terminal repeat (LTR) sequences from circularized viral DNA of the three isolates