Figure 5

NSP4 binds to cells of distinct lineage. A, Cells were incubated with 5 μg/ml NSP4 and binding measured as described in the text. A representative histogram from three independent experiments is shown. Shaded peak indicates background fluorescence of cells stained with primary and secondary antibody in the absence of NSP4. B, Human peripheral blood mononuclear cells (PBMC) were incubated with 5 μg/ml NSP4 and binding measured as described in the text. The PBMC were stained with antibodies directed against CD3, CD20 and CD14 to identify T cell, B cell and monocyte subsets respectively