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Figure 1 | Virology Journal

Figure 1

From: Rotavirus NSP4 is secreted from infected cells as an oligomeric lipoprotein and binds to glycosaminoglycans on the surface of non-infected cells

Figure 1

Purification of NSP4 from culture medium of rotavirus infected Caco-2 cells. NSP4 was purified by concanavalin A affinity chromatography and cation exchange (Mono S) chromatography. A, Purification was monitored by SDS PAGE and silver staining of electrophoresed proteins Lane 1, M.W. markers; 2, concentrated media post centrifugation; 3, protein eluted from conA in 1 M α-methyl mannoside; 4, Mono S eluate (1 M NaCl). B, Elution profile of material loaded on Mono S column is shown in B. C, Western blot of purified rotavirus TLPs (2.5 μg) and purified NSP4 3 μg probed with a polyclonal antiserum (D37) raised against purified SA11 rotavirus

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Virology Journal

ISSN: 1743-422X