Figure 4

Neonates had reduced expression of IFN-γ, TNF-γ and CXCL2 even at higher doses of virus. Adult (n = 3 for each dose) and neonatal C57BL/6 mice (n = 6 for each dose) were infected with 50, 500, 5000, or 50,000 pfu SeV/g body weight. a) Body weight loss in adults b) Body weights in neonates. White circles 50 pfu/g body weight, black circles 500 pfu/g, white squares 5,000 pfu/g, and black squares 50,000 pfu/g. c) Adult survival graph: white circles 50 pfu/g (n = 6), black circles 500 pfu/g (n = 6), white squares 5,000 pfu/g (n = 6) black squares 50,000 pfu/g (n = 6). d) Neonatal survival graph: white circles 50 pfu/g (n = 8), black circles 500 pfu/g (n = 9), white squares 5,000 pfu/g (n = 8) black squares 50,000 pfu/g (n = 7). e) Post-infection day 7 IFN-γ protein expression was determined by ELISA. *p < 0.05. f) Post-infection day 7 TNF-α protein expression was determined by ELISA. TNF-α proteins levels were below the level of detection in the neonatal samples at inoculation doses of 500, 5000, and 50,000 pfu/g body weight (*none detected). g) Post-infection day 7 CXCL2 protein expression was determined by ELISA. Adults (white bars); Neonates (black bars). Data are means ± SD.