Figure 5

PARP10/NS1 expression level could change cell cycle of A549. a. Total RNA was extracted from transfected cells, and NS1 and PARP10 transcription level were identified by RT-PCR. PARP10 siRNA could significantly reduce PARP10 transcription level; NS1 transient expression had less inhibition on PARP10, while PARP10 expression vector could effectively express the target proteins. b. Transfect cells was analyzed by flow cytometry, and it was found that when NS1 transient expression and PARP10 knock-down were performed together, the percentage of A549 cells in G2-M stage grew to 45% from 10%. When PARP10 expression level was elevated, the percentage of cells in G2-M stage saw significant decrease, similar to the percentage of cells transfected with empty vector, but the percentage of cells in G1-S stage grew from less than 10% to 20%.