Figure 4

NS1 of high expression in A549 cells could reduce endogenous PARP10 expression. a. NS1 of H5N1 AIV was transiently expressed in A549 cells, which were then lysed with RIPA buffer, and examined by Western blotting using anti-PARP10 monoclonal antibody, with β-actin as internal control. It was found that NS1 could reduce PARP10 expression level. b. RT-PCR assay found that with GAPDH as internal control, PARP10 saw a low RNA expression level because of the high expression of NS1.