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Figure 2 | Virology Journal

Figure 2

From: The interaction between the PARP10 protein and the NS1 protein of H5N1 AIV and its effect on virus replication

Figure 2

NS1 interacting C-terminal of PARP10 identified with co-immunoprecipitation. a. The schematic domain architecture of whole/truncated PARP10. PARP10 protein was divided into four fragments according to their corresponding domains: RRM domain and glycine rich region (aa 1-500), glycine rich region and glutamate rich domain (aa 270-697), glutamate rich region and PARP domain (aa610-1025), and PARP domain (aa 691-1025). Nuclear export signal and two ubiquitin-binding motifs are located at the overlapping area of glutamate rich region and PARP domain (aa632-697). b. The whole/truncated PARP10 and Flag-NS1 were transiently expressed in A549 cells, which were lysed in RIPA buffer, co-immunoprecipitated using anti-Myc antibody or anti-Flag antibody, and the sediment obtained was examined by Western blotting. One tenth of each lysate was taken to identify protein expression.

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