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Figure 1 | Virology Journal

Figure 1

From: The interaction between the PARP10 protein and the NS1 protein of H5N1 AIV and its effect on virus replication

Figure 1

NS1 could have in vitro and in vivo interaction with PARP10. a. Interaction of GST-NS1 and Myc-PARP10 were identified in GST-pulldown assay. Bacterial expresssed soluble GST and GST-NS1 protein were purified, and SDS-PAGE and Commassie Blue Fast Staining revealed that GST and GST-NS1 of higher purity were obtained. There was a stripe similar to the size of GST under GST-NS1 stripe, indicating that GST-NS1 degraded during the purification. Myc-PARP10 was transient expressed in A549 cells and identified by immuno-blotting. The sediment of GST-pulldown was examined by immno-blotting using anti-Myc antibody. It was found that GST-NS1 could capture Myc-PARP10, while GST could not. b. NS1 could have in vivo interaction with PARP10. Myc-PARP10 and Flag-NS1 were transiently expressed in A549 cells, which were lysed in RIPA buffer, co-immunoprecipitated using anti-Myc antibody or anti-Flag antibody, and the sediment obtained was examined by Western blotting. One tenth of each lysate was taken to identify protein expression.

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Virology Journal

ISSN: 1743-422X