Serum IgG Ab titers were detected by ELISA. Nine mice were immunised with 200 μg multi-mimotope. The mice were bled once (unimmunized serum), and were bled every 10 days after the booster immunization (immunized serum). Ten days after the booster injection, mice were challenged with H1N1 virus by intranasal inoculation of 50 μl per mouse. Two weeks after challenge, mice were sacrificed for the anti-H1N1 IgG Ab detection by ELISA (immunized infected serum). Triplicate ELISAs were performed to test each serum sample. All assays were carried out in triplicate and the error bars indicate standard deviation.