Table 1 Primers used in this study
From: Construction and characterization of an infectious clone of coxsackievirus A16
Primer | Sequence (5' - 3') | Enzyme site | Purpose |
|---|---|---|---|
P1 | GCCAAGCTT AAAACAGCCTGTGGGTTGTTCCCACCC | Hind III | CV(1-4392) amplification |
P2 | CGGGTCTAGA GCGTAGACTCTTTTGGCTTCAGTC | Xba I | CV(1-4392) amplification |
P3 | CTACGCTCTAGA AAGAAGGA | Xba I | CV(4381-7410) amplification |
P4 | ACAAGCGGCCGC TGCTATTCTGGTTATAAC | Not I | CV(4381-7410) amplification |
P5 | CTTCTCGAG GTTGATTTTGAGCAAGCATTG | Xho I | CV(6087-7410-pA) amplification |
P6 | TATGCGGCCGC TTTTTTTTTTTTTTTTTTTTTTTTT | Not I | CV(6087-7410-pA) amplification |
P7 | CTAAAGCTT AGCTAATACGACTCACTATAGTTAAAACAGCCTGTGGGTTG | Hind III | T7 promoter introduction/priming cDNA synthesis from negative-strand RNA |
P8 | CCTATTGCAGACATGATTGACCAG | none | RT-PCR for negative-strand RNA |
P9 | TGTTGTTATCTTGTCTCTACTAGTG | none | RT-PCR for negative-strand RNA |