Figure 6

Immunofluorescence staining of cells infected with the R1 virus or the wild-type virus. Infected cells were incubated with polyclonal guinea pig anti-CVVP0 (A-C and M-O), anti-CVVP1t (D-F and P-R), anti-CVVP3 (G-I and S-U), or pre-immune serum (J-L and V-X), followed by incubation with a FITC-conjugated goat anti-guinea pig IgG antibody. Cells were also stained with DAPI. (A, D, G, J, M, P, S and V) images captured using a FITC filter; (B, E, H, K, N, Q, T and W) images captured using a DAPI filter; and (C, F, I, L, O, R, U and X) merged images.