Figure 4

Detection of negative-sense RNA by RT-PCR. RNA extracted from transfected or infected cells was subjected to reverse transcription with the primer P7 and subsequent PCR amplification of an 882-bp fragment using primers P8 and P9. Lane M, DNA marker; lane 1, PCR product of mock-transfected cells; lane 2, PCR product of RNA-transfected cells; lane 3, PCR product of wild-type virus infected cells.