Figure 1
From: Construction and characterization of an infectious clone of coxsackievirus A16
Construction of a full-length infectious clone of CVA16. (A) PCR amplification of CVA16 specific fragments. Lane M, DL5000 DNA marker (TaKaRa Biotechnology, Dalian, China); lane 1, CV(1-4392); lane 2, CV(4381-7410); and lane 3, CV(6087-7410-pA). (B) PCR amplification of the CVA16 full-length cDNA plus T7 promoter and 3' poly(A) sequence. Lane M, DL15000 DNA marker (TaKaRa Biotechnology, Dalian, China); lane 1, T7-CV(1-7410-pA) amplicon. (C) Schematic representation of the plasmid pMD19-CV. T7, T7 promoter; CV(1-7410), nucleotides 1-7410 of the CVA16 genome; pA, poly(A) sequence.