Figure 1From: Downregulation of APOBEC3G by xenotropic murine leukemia-virus related virus (XMRV) in prostate cancer cellsDetection of A3G in prostate cancer cells of epithelial origin A3G was detected using two different polyclonal rabbit sera obtained from the NIH AIDS Reagent Program. Anti-ApoC17 was raised against a synthetic peptide comprising of the 17 C-terminal residues of A3G (Cat. No. 10082), while the other was (Anti-ApoC29) Anti-A3G C-terminal antisera raised against a C-terminal peptide representing the last 29 amino acids of human A3G coupled to a hapten (Cat. No 10201). As a loading control β-actin (Sigma Co., USA) was used. For immunoblot analysis, cell lysates (5 μg) were subjected to SDS-polyacrylamide gel electrophoresis and western blot analysis with the appropriate antibodies. Western blot analysis using, (A) Anti-ApoC29 and (B) Anti-ApoC17.Back to article page