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Table 1 Real-time PCR quantification of different target sequences in virus isolates obtained by the marker rescue procedure

From: Selection of recombinant MVA by rescue of the essential D4R gene

Template Total MVA(1) YF prME(1) lacZ(1) AmpR(1)
rMVA-YF/D4 #1 8.54 8.44 3.33 3.83
rMVA-YF/D4 #2 8.38 8.25 3.17 4.02
rMVA-YF/D4 #3 8.54 8.30 3.47 4.02
rMVA-YF/D4 #4 8.58 8.24 3.37 4.07
rMVA-YF/D4 #5 8.69 8.42 3.37 3.89
rMVA-YF/D4 #6 8.33 8.14 3.45 4.08
rMVA-YF/D4 #7 8.64 8.49 3.66 4.11
rMVA-YF/D4 #8 8.54 8.50 3.61 4.02
rMVA-YF/D4 #9 8.74 8.55 3.48 3.97
rMVA-YF/D4 #10 8.39 8.27 3.54 4.07
rMVA-YF/D4 #11 8.75 8.49 3.42 3.96
rMVA-YF/D4 #12 8.46 8.44 3.56 4.06
Uninfected cDF-1 0.00 0.00 3.58 4.01
Wild-type MVA 8.41 2.62 3.27 3.93
dMVA-ZG 7.46 3.57 7.34 3.93
  1. (1)Results are given in log10 genomic equivalents per ml, and are means of duplicate measurements