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Figure 2 | Virology Journal

Figure 2

From: Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice

Figure 2

PAGE analysis of the culture supernatants under denaturing and native conditions demonstrating the secreted expression of recombinant HA protein as HA monomers and HA trimers respectively. Panel 2A: Methanol induced culture supernatants under denaturing conditionLane M: Pre-stained Protein marker (Fermentas, USA, #SM 0671) Lane 1: Cell culture supernatant from Pichia multiple copy integrant Clone A Lane 2: Cell culture supernatant from Pichia multiple copy integrant Clone B Lane 3: Cell culture supernatant from Negative clone Panel 2B: Methanol induced culture supernatants under native conditionLane M: Pre-stained Protein marker (Fermentas, USA, #SM 1811) Lane 1: Cell culture supernatant from Pichia multiple copy integrant Clone B Lane 2: Cell culture supernatant from Pichia multiple copy integrant Clone A Lane 3: Cell culture supernatant from Negative clone.

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