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Figure 1 | Virology Journal

Figure 1

From: Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice

Figure 1

Vector map of the recombinant construct and Schematic diagram of genetic recombination in Pichia. Panel 1A: Plasmid map of recombinant yeast transfer vector pPIC9KH1N1HA. Panel 1B: Schematic diagram showing the genetic recombination event that result in the formation of Pichia transformant with multiple-copy integrants(The gene of interest (H1N1HA) is positioned between Eco RI and Not I sites under the control of AOX1 promoter, the secretory signal (SS), transcription termination (TT) signal sequences are on 5' and 3'end of the H1N1HA gene respectively. HIS 4 locus is carrying the Sal I recognition sequence and the integration of the transgene within the Pichia genome will be at His 4 locus).

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Virology Journal

ISSN: 1743-422X