Sequence similarity between the erythrocyte binding domain of the Plasmodium vivax Duffy binding protein and the V3 loop of HIV-1 strain MN reveals a functional heparin binding motif involved in binding to the Duffy antigen receptor for chemokines

Table 1 Heparin-Sepharose peak fractions of peptides of the V3 loop of HIV, hbs-wt and hbs-kara of the P. vivax DBP, and RANTES

Peptide	Amino Acid Sequence	Charge @ pH 7.0	[NaCl] of Peak Fraction
Cyclic V3 strain MN	CTR PNYNKRKR IDIGPGR AFTTK NIIGTIR QAHC	+7	0.5 M
Linear sub-V3 strain MNa	CTR PNYNKRKR IHIPGPGR A	+6	1.0 M
Linear sub-V3 strain MNb	R IHIGPGR AF YTTK NIIGTI	+3	0.15 M
Linear sub-V3 strain MNc	YTTK NIIPTIR QAHCNISR A	+3	0.15 M
Cyclic V3 subtype EA	CTSITIGPGQVFYR TGC	+1	0.15 M
Cyclic V3 Subtype B	CK GIR IPGPR AVYAAEC	+2	0.15 M
Linear V3 Strain RF	K SITK GPGR VIYATG	+3	0.15 M
Linear V3 Strain SF2	K SIFIGPGR AFHTTG	+2	0.15 M
Linear V3 Strain IIIB	TR PNNNTRK SIR IQR GPGR AFVTIGK IGNMR	+8	0.5 M
Hbs-wt	NCNYKRKRR ER DWDCNDYK DDDDK	0	0.5 M
Hbs-kara	NCNYA RK A R EA DWDCNDYK DDDDK	- 3	0.01 M
RANTES	MK VSAAR LAVILIATACAPASASPYSSDTTP
	CCFAYIAR PLPR AHIK EYFYTSGK CSNPAVV
	FVTRK NR QVCANPEKK WVR EYINSLEMS	+7	0.5 M

ISSN: 1743-422X