Heparin inhibition of region II of P. knowlesi EBPs binding to rhesus erythrocytes. The P. knowlesi alpha, beta and gamma proteins are members of the DBP family, bind rhesus erythrocytes by region II, and contain a consensus HBM. Only the alpha protein binds DARC. The PvRII binding assay shown in Figure 2 was repeated using plasmids to express the P. knowlesi EBPs, and using rhesus erythrocytes. Inhibition was determined in the same manner as the PvRII binding assay.