Figure 3

Polyanion inhibition of P. knowlesi invasion of DARC+ erythrocytes. The same polyanions used to inhibit the pvRII binding assay shown in Figure 2 were used in the P. knowlesi invasion assay. The inhibition of invasion was determined by subtracting the number of chemokine-treated DARC+ human erythrocytes invaded by P. knowlesi merozoites (per 2000 erythrocytes) from the number of untreated DARC+ human erythrocytes invaded by P. knowlesi merozoites, and dividing by the number of untreated, invaded erythrocytes.