Figure 2

The result of ELISPOT, FCM and ELISA of the spleen lymphocyte in BALB/c mice (mean ± SD). The application of aluminium have statistical significance of the population of the CD19⁺CD27⁺ B cell and CD27⁺CD38⁺cell. (P < 0.01, P < 0.0001). The population of the CD19⁺CD38⁺ B cell of Alum+CpG to the PBS group have statistical significance.(P < 0.05). The concentration of IL-6, IL-10 in cultivated supernatant of the splenic lymphocytes of the BALB/c mice after the incentivation of HCV antigen in vitro(mean ± SD)The concentration of IL-6 and IL-10 was determined by ELISA from the four groups of four mice and single-cell suspensions were prepared. After lysing red blood cells with Tris-NH₄Cl, splenocytes (5 × 10⁶ cells in 200 ml) were cultured at 37°C withE1+HVR1 recombination antigen (20 μg/ml)or LPS(1 μg/ml)in vitro, using the ELISA assay to measure the titer of IL-6 and IL-10. Results are shown as the mean value obtained for duplicate wells.