Vaccinia titres in immunised mice challenged with rTTV-CE1E2. Mice were sacrificed 5 days post-challenge. Vaccinia titres in ovaries were determined. Mice (five or six per group) received a single immunisation (Figure 5A). Mice (six per group) received two immunisations and were challenged after the second (booster) immunisation (Figure 5B). The data are presented as the mean with SEM of each group. Significant p values between the vaccinated groups are shown. * indicates p < 0.05, ** indicates p < 0.01.