Figure 3

Cellular immune responses in rAd5-CE1E2 immunised mice. An ELISPOT assay of splenocytes after stimulation with an HCV peptide pool representing the core, E1, or E2 proteins. Splenocytes were isolated at weeks 2 (A, post-prime), 5 (B, post-boost), and 20 (C, long-term). The data are expressed as spot-forming cell (SFC) responses to different peptide pools, and are presented as means with SEM of six mice per group. Significant p values between the vaccinated groups are shown. Control group mice injected with PBS generated SFC responses of < 20 per 106 mononuclear cells. Significant p values between vaccinated groups are shown. * indicates p < 0.05, ** indicates p < 0.01.