Table 1 Summary of primer sequences encompassing the EBV types and variants
From: Characteristics of epstein barr virus variants associated with gastric carcinoma in Southern Tunisia
EBV genes and regions (types or variants) | Primer sequences (5'-3') | Product size (bp) |
|---|---|---|
EBNA-3C gene (type A or B) | F: AGAAGGGGAGCGTGTGTTGT R: GGCTCGTTTTTGACGTCGGC | PCR products 153: type A 246: type B |
BamH1-W region (type C or D) | F: ACCTGCTACTCTTCGGAAAC R: TCTGTCACAACCTCACTGTC | PCR+ Bam HI digestion 205: type C 130+75: type D |
BamH1-F region (f variant) | F: TCCCACCTGTTACCACATTC R: GGCAATGGGACGTCTTGTAA | PCR+ Bam HI digestion 198: F variant 127+71: f variant |
Exon 1 of BNLF1 gene ( XhoI variant) | F: ACAATGCCTGTCCGTGCA R: AGAAACACGCGTTACTCT | PCR+ Xho I digestion 497: Xho I- 340+157: Xho I+ |
Exon 3 of BNLF1 gene (30bpdel-LMP1) | F: TGGAGGGAGAGTCAGTCAGGC R: ATTGACGGAAGAGGTTGAAAAC | PCR products 224: 30bpdel-LMP1 254: wt-LMP1 |