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Table 2 Oligonucleotide primers used in the production of the RNAi construct

From: RNA interference-based resistance against a legume mastrevirus

Primer

Orientation

Primer sequences#

Cloning sites*

Chp6SF

sense/forward

5'CCAACTCGAG TTATCAAGCTGGACAAGAGC 3'

Xho I

Chp6SR

sense/reverse

5'CCTCCCATGG TTCGCCTTAAACAGAAGG 3'

Nco I

Chp6ASF

antisense/forward

5'CCTCTCTAGA TTCGCCTTAAACAGAAGG 3'

Xba I

Chp6ASR

antisense/reverse

5'CCAATCTAGA TTATCAAGCTGGACAAGAGC 3'

Xba I

  1. * Restriction endonuclease recognition sequences introduced into the primers to facilitate cloning of fragments into pFGC5941.
  2. # The introduced restriction endonuclease recognition sequences are underlined.
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Virology Journal

ISSN: 1743-422X