Figure 5
From: Site directed biotinylation of filamentous phage structural proteins
Selective affinity depletion of serum using biotinylated phages. Phages "12mer" and "5mer" express two epitopes of HIV (12mer: CWGGTNWGDTPIVC, 5mer: CSGKLIC). The total activity of HIV+ polyclonal serum is shown in strip #1. The serum was applied to 5mer-phages biotinylated on Protein 7 and bound to streptavidin magnetic beads. Strip #2 illustrates the affinity purified antibodies specific for the 5mer eluted off the column. Strip #3 shows the depleted serum after passing over the 5mer column. As a control the columns were prepared using the same 5mer phages but not biotinylated at all. Strip #4 shows that in this situation practically no antibody can be eluted off the column and all the activity passes through the column as is shown in strip #5.