Figure 4

Production and biotinylation of multi-functional phages. Phages were produced either without any insert (fth1), or containing epitopes for mAbs 4H3 or CG10, both antibodies directed against HIV gp120. The three phages were then used to infect E. coli bacteria that had been transformed with either pUC18 expressing a Protein 8 fusion with the CG10 epitope or with BT as indicated. Three identical dot-blots of the phages generated from these transformed cells were probed with mAbs 4H3, CG10 or with Streptavidin-HRP (SA). All the phages derived from the BT-transformed bacteria express biotinylated Protein 8 as well as the other corresponding markers, thus illustrating the possibility for multi-functionality of the phages.