Figure 3
From: Site directed biotinylation of filamentous phage structural proteins
Biotinylation in vivo and in vitro of phage proteins 3, 7, 8 and 9. The BT was introduced into the phage proteins 3, 7, 8 and 9 and compared with fth1 phage as indicated. The phages were tested by quantitative ELISA for the presence of biotin with (stipple and black) or without (grey and hatched) lac-i modified pBIRAcm for in vivo biotinylation (grey and stipple) compared with subsequent in vitro biotinylation (hatched and black).