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Table 4 Infectivity of pools of agroinoculable ternary Yeast-Escherichia coli-Agrobacterium tumefaciens shuttle vectors containing the ACLSV FL-cDNA under the control of the 35S promoter assembled by one-step homologous recombination-based cloning in yeast

From: Strategies to facilitate the development of uncloned or cloned infectious full-length viral cDNAs: Apple chlorotic leaf spot virus as a case study

Plasmid poolsa

Infected/biolistically inoculatedb

Infected/Agroinoculatedc

Infectious plasmids per pool

 

C. quinoa

C. quinoa

N. occidentalis 37B

 

1

0/6

0/6

0/5

0

2

0/6

0/6

0/5

0

3

3/5

4/6

2/4

1

  1. a: each pool was composed of 12 recombinant plasmids showing the expected restriction mapping results
  2. b: biolistic inoculation was performed using two shots par plant and 100 ng of the pool of purified plasmids per shot (about 8 ng of individual plasmid per shot).
  3. c: Agroinoculation was performed by infiltrating two leaves of each plant with the suspension of agrobacterium cells.
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Virology Journal

ISSN: 1743-422X