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Table 2 Infection rates of two ACLSV hosts following mechanical inoculation of in vitro transcripts obtained from ACLSV FL-cDNA under the T7 promoter synthesized using two different PCR enzymes

From: Strategies to facilitate the development of uncloned or cloned infectious full-length viral cDNAs: Apple chlorotic leaf spot virus as a case study

Enzyme used for LD-PCR

Infected/inoculated plants (% infected)a,b

 

Chenopodium quinoa

Nicotiana occidentalis 37B

Advantage GCc

8/36 (22%)

0/22 (0%)

Phusiond

30/36 (83%)

0/22 (0%)

water inoculation controle

0/12 (0%)

0/12 (0%)

  1. a: the results shown are the sum of two inoculation experiments performed with transcripts deriving from PCR products resulting from different amplification reactions.
  2. b: plants were mechanically inoculated using 5 μg of transcripts per plant.
  3. c: Advantage® GC Genomic LA Polymerase Mix (Clontech)
  4. d: Phusion® High Fidelity DNA Polymerase (Finnzyme)
  5. e: plants were rub inoculated using the DEPC-treated sterile water used to resuspend the in vitro transcripts.