Figure 5
From: Hepatitis B virus infection and replication in human bone marrow mesenchymal stem cells
Southern blot analysis of HBV cccDNA in infected human BMSCs. (A) HBV cccDNA was extracted from human BMSCs at 2, 4, 6, and 8 days after infection and subjected to Southern blot hybridization. DIG-labeled whole HBV DNA probe served as a positive control (lane M). Uninfected BMSCs served as a negative control (lane U). DNA sizes are indicated on the left (kb). (B) The cccDNA cleaved by E coRI was analyzed using Southern blot analysis.