Figure 4

NS1-C antagonises IFN response in the absence of an RNA stimulus. HEK-293TN cells were transfected with IFN-β luciferase reporter plasmid, plasmids encoding mutant RIG-I (RIG-I-N) and β-galactosidase, and vectors encoding full-length or truncated NS1 from C/JJ/50 or C/JHB/1/66, or empty pCI vector. Relative luciferase activities of cell lysates 32 hours post transfection were determined. Error bars indicate standard deviations.