Table 3 Oligonucleotide primers used in this study
From: Reactions of Nicotiana species to inoculation with monopartite and bipartite begomoviruses
Primer | Sequence | Predicted size of amplification product (base pairs) |
|---|---|---|
RDRf | CGAGCCAGTTGCGGGATAATTC | 269, 341 |
RDRr | GAGCAAAGTCAGCAGATATT | Â |
CLCV1 | CCGTGCTGCTGCCCCCATTGTCCGCGTCAC | 1102 |
CLCV2 | CTGCCACAACCATGGATTCACGCACAGGG | Â |
PK3AV2F | AAATATCGATATGTGGGATCCACTATTAAACG | 311 |
PK3AV2R | TCTGGTCGACCTATACATGGGCCTGTTTGT | Â |
BegomoF | ACGCGTGCCGTGYTGCTGCCCCATTGTCC | ~2800 |
BegomoR | ACGCGTATGGGCTGYCGAAGTTSASACG | Â |
ToLCNDV2F | GTCGACAAACATGTGGGATCCATTATTGC | 352 |
ToLCNDV2R | ATCGATCTTCTATACATTCTGTACATTC | Â |
TLCNDV1 | GCAGATATCATCATTTCAACGC | 642 |
TLCNDV2 | CATACTTGCCGGCCTCTTGTTG | Â |