Figure 7

PCR-mediated detection of ToLCNDV in inoculated Nicotiana plants. The ethidium bromide-stained agarose gel was photographed under UV illumination. The samples loaded on the gel resulted from PCR reactions with primer pair ToLCNDV2F/ToLCNDV2R and DNA extracted from the leaves of plants (as indicated above each well) inoculated with ToLCNDV. The leaves sampled were developing at the time of, or developed after, inoculation and were sampled at 25 dpi. The presence of a 352 bp band indicates the systemic movement of ToLCNDV from the site of inoculation. The sample in lane C resulted from PCR amplification with DNA extracted from a healthy N. benthamiana plant. A DNA size marker was electrophoresed in lane M. The sizes (bp) of selected marker bands are indicated on the left.