The development of a rapid SYBR Green I-based quantitative PCR for detection of Duck circovirus

Table 2 Comparison of real-time PCR and conventional PCR assay results

Conventional PCR assay results		real-time PCR assay results
		Positive	Negative	Total
Positive		9	0	9
Negative	20	7	13	20
	45^#	0	45	45
Total		16	58	74

^# These 45 samples described at Materials and methods, detection of clinical samples for conform that whether the DuCV can be transmitted vertically. And also no positive was shown by the optimized real-time PCR.

ISSN: 1743-422X