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Table 1 Intra- and inter-assay reproducibility of the real-time PCR

From: Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus

Standard plasmids

Copies

Intra-assay (C T Value)

Inter-assay (C T value)

  

Mean

SD

CV(%)

Mean

SD

CV(%)

1

5.0 × 103

28.09

0.25

0.89%

28.00

0.20

0.72%

2

5.0 × 104

24.56

0.15

0.61%

24.68

0.14

0.57%

3

5.0 × 105

21.74

0.09

0.42%

21.90

0.21

0.97%