From: Establishing a TaqMan-Based Real-Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus
Standard plasmids
Copies
Intra-assay (C T Value)
Inter-assay (C T value)
Mean
SD
CV(%)
1
5.0 × 103
28.09
0.25
0.89%
28.00
0.20
0.72%
2
5.0 × 104
24.56
0.15
0.61%
24.68
0.14
0.57%
3
5.0 × 105
21.74
0.09
0.42%
21.90
0.21
0.97%