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Figure 4 | Virology Journal

Figure 4

From: Epstein - Barr virus latent membrane protein 1 suppresses reporter activity through modulation of promyelocytic leukemia protein-nuclear bodies

Figure 4

siRNA targeted disruption of PML NBs restores MMP9 reporter activity. A) A549 cells retrovirally transduced with either the backbone or LMP1 expression vector were transfected with either control siRNA or siRNA directed towards the PML transcript. PML NB intensity (green) was assessed at 24 hours post transfection. LMP1 is shown in red, DAPI nuclear counter-stain is shown in blue; original magnification of 400X. B) 7.5 × 104 A549 cells were reverse transfected with either the control siRNA or siRNA directed towards the PML transcript at plating. After 24 hours, the cells were co-transfected with 100 ng of the indicated luciferase construct and 300 ng of LMP1 expression vector. Cells were harvested at 24 hours post transfection and luciferase activity assessed.

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Virology Journal

ISSN: 1743-422X