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Figure 3 | Virology Journal

Figure 3

From: Epstein - Barr virus latent membrane protein 1 suppresses reporter activity through modulation of promyelocytic leukemia protein-nuclear bodies

Figure 3

Arsenic trioxide restores expression of exogenous DNA. A) ATO treatment disrupts PML NB. Retrovirally transduced A549 cell expressing LMP1 or a hygromycin resistance cassette (control) were treated for 24 hours with the indicated concentration of ATO and stained for PML (green). Nuclei are stained with DAPI (blue) at original magnification of 600X. B) ATO restores luciferase activity in a dose responsive manner. 7.5 × 104 A549 < LMP1> Hi cells were transfected with 100 ng NF-kB responsive luciferase and treated with the indicated concentration of ATO for 24 hours. C) LMP1 is differentially expressed in separate retroviral transductions. A549 cells were retrovirally transduced with backbone vector expressing only the hygromycin resistance cassette or LMP1 expression vector. Western blot analysis of whole cell lysate of control cells and two separate LMP1 vector transductions; 35 ug of protein was loaded per well. Beta actin was used as a loading control. D) ATO-induced restoration of exogenous DNA expression is LMP1 dose responsive. 7.5 × 104 A549 cells retrovirally transduced with backbone vector or 2 separate retroviral transductions differentially expressing LMP1 were transfected with pGL3_Luc and treated with 8 nM ATO for 24 hours.

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