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Figure 2 | Virology Journal

Figure 2

From: Epstein - Barr virus latent membrane protein 1 suppresses reporter activity through modulation of promyelocytic leukemia protein-nuclear bodies

Figure 2

LMP1 suppression of reporter activity is dose responsive. 7.5 × 104 A549 cells were co-transfected with 100 ng of A) an NF-kB responsive reporter or B) the constitutive reporter pGL3-comtrol and the indicated amount of the LMP1 expression vector. The total amount of DNA in each well was held constant by addition of empty backbone vector. C & D) In a dual luciferase assay, 7.5 × 104 A549 cells were co-transfected with the TopFlash firefly and the Thymidine Kinase renilla luciferase construct and the indicated amount of the LMP1 expression vector. 10 mM LiCl was used as a positive control. Cells were harvested at 24 hours post transfection. E) Top-Flash luciferase activity normalized to RL-TK expression.

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