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Table 1 Primers used to construct the full-length cDNA clone and helper plasmids for Flury LEP

From: Generation of a recombinant rabies Flury LEP virus carrying an additional G gene creates an improved seed virus for inactivated vaccine production

Purpose

Name

Primer (5'-3')a

For F1 amplification

F1-F

TGCGCTAGC TGTTAAGCGTCTGATGAGTCCGTGAGGACGAAACTATAGGAAAGGAATTCCTATAGTC ACGCTTAACAACAAAACCAAAGAAb

 

F1-R

GGCACGCGT ACTCCACATAACTTGAGTTTGC

For F2 amplification

F2-F

AGGCCTGTATAAGTCTTTAAAGGGAGCA

 

F2-R

ATCGGGGTTCCCGGCCTCTTGACACAAC

For F3 amplification

F3-F

TATGCTAGC TCTGGTTAAGCTCCCACGAATC

 

F3-R

CGATCCCGGG ccccgcgggggcccctcccttagccatccgagtggacgaacgtcctccttcggatgcccaggtcggaccgcgaggaggtggagatgccatgccgacccACGCTTAACAAATAAACAATc

For Pme I mutation

Pmu-F

GACTTGAAGTT TA AAC AGGATGACCGGCCd

 

Pmu-R

GGCCGGTCATCCTG TTT AA ACTTCAAGTC

For G gene amplification

G1

ATGCGTTTAAAC AAGTTTATCACTTGTTTACCTCT

 

G2

GCATGTTTAAAC ACTTGAAGTGTCAAAAGGATGA

For N gene amplification

HN-F

GGCGAATTC ATGGATGCCGACAAGATTGT

 

HN-R

CCGGGTACC TTATGAGTCACTCGAATACG

For L gene amplification

HL-F

GGCGAATTC ATGCTGGATCCGGGAGAGGTTT

 

HL-R

CCGGGTACC TTACAAACAACTGTAGTCTA

For LEP-G confirmation

G3

ATGCTTTCTCTTGAATGTGG

 

G4

GGGTTTGGAAAAGCATATAC

  1. a Restriction enzyme sites are shown in boldface. b The HamRz sequence is shown in italics. c The HdvRz sequence is shown in lowercase. d The nucleotides that were changed are underlined.