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Figure 2 | Virology Journal

Figure 2

From: Generation of a recombinant rabies Flury LEP virus carrying an additional G gene creates an improved seed virus for inactivated vaccine production

Figure 2

Confirmation of recovery of the rLEP-G from cloned cDNA. (A) RT-PCR, with the G3 and G4 primers, detected the genome RNA from rLEP-G after two passages in BHK-21 cells (lane 1), LEP (lane 2), and rLEP-G after 10 passages in BHK-21 cells (lane 3). (B) The RT-PCR amplified cDNA fragment was digested with Pme I. M, molecular weight marker. (C) Western blot analysis of G protein expression in BHK-21 cells infected with viruses. Cells were collected at 96 h post-infection with LEP or rLEP-G and subjected to Western blot analysis as described in the Methods. The G/β-actin ratio was calculated by dividing the densitometry of the G protein band by that of the β-actin protein band.

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Virology Journal

ISSN: 1743-422X