Skip to main content
Figure 1 | Virology Journal

Figure 1

From: Generation of a recombinant rabies Flury LEP virus carrying an additional G gene creates an improved seed virus for inactivated vaccine production

Figure 1

Schematic representation of recombinant virus containing two homologous G genes. The pLEP vector was obtained by assembling three overlapping cDNA fragments of the Flury LEP strain into the vector pCI, as described in the Methods. The 1801-bp fragment including the G gene ORF was introduced into the LEP genome through the Pme I site in the G-L noncoding region. The hammerhead ribozyme sequence (HamRz) was introduced upstream of the LEP genome, and the hepatitis delta virus ribozyme sequence (HdvRz) was introduced downstream of the viral genome.

Back to article page