Figure 5
From: Effect of ultrasound on herpes simplex virus infection in cell culture
Effect of ultrasound on the viability of SAS cells. (A) SAS cell suspension was exposed to ultrasound in the presence or absence of microbubbles. Cells were incubated in 96-well plates for 48 h and cell viability was measured by MTT assay. The duty cycle and exposure time were fixed to 10% and 20 sec and output intensity was changed from 0.5 to 2.0 W/cm2, according to Sonitron intensity dial settings. (B) The output intensity and exposure time were fixed to 0.5 W/cm2 and 20 sec and duty cycle output was changed from 5 to 50%. (C) The output intensity and duty cycle were fixed to 0.5 W/cm2 and 10% and exposure time was changed from 10 to 40 sec. The values were divided by that of the control and the percentage was calculated. Data are means ± SDs of three determinations. *P < 0.05 vs. control.