Figure 3

Effect of ultrasound on the formation of plaques by HF in Vero cells, and by R849 in SAS cells. (A) Vero cells were inoculated with 50 PFU of a fusogenic HSV-1 mutant HF and cultured at 37°C for 30 min. Thereafter, cell monolayers were exposed to ultrasound in the presence or absence of microbubbles as described in Figure 2. After further incubation, the number of plaques was determined. (B) SAS cells were inoculated with 100 PFU of R849 and cultured for 30 min. They were treated as described in (A). (C) Vero cells grown in lumox ™ multiwell plates were inoculated with 100 PFU of R849 and cultured for 30 min. They were treated as described in (A). Data are means ± SDs of three determinations. *P < 0.05.