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Figure 5 | Virology Journal

Figure 5

From: Expression of an IRF-3 fusion protein and mouse estrogen receptor, inhibits hepatitis C viral replication in RIG-I-deficient Huh 7.5 cells

Figure 5

Expression of 1-8U and hnRNP M genes in Huh7.5-IRF3ER cells. A. Gene expression of 1-8U in Huh7.5-IRF3ER cells. Huh7.5-IRF3ER cell lysates were harvested at 0, 24, and 48 hours after adding 4-HT to induce IRF-3ER dimerization. Total protein was extracted and analyzed by Western blotting with an anti-1-8U antibody. Lane 1, Huh7.5-IRF-3ER cell lysate from cells treated with 4-HT at 0 hours; lane 2 and 3, Huh7.5-STAT1ER cell lysates from cells treated with 4-HT for 24 and 48 hours. Actin protein was used as an internal control. B. Expression of hnRNP M in Huh7.5-IRF3ER cells. Huh7.5-IRF3ER cells were treated by 4-HT (1 μM) for 72, 48, and 24 hours prior to collecting cell lysates. Control is mock treatment of 4-HT for 72 hours. Expression of hnRNPM and GAPDH (used as internal control) mRNA was measured using Taqman primers from Applied Biosystems in triplicate. Relative hnRNP M RNA levels were calculated as a proportion of the control (1.0).

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